Not known Facts About attune flow cytometer

Not known Facts About attune flow cytometer

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Info-enriched characterization of mobile populations. Morphological details from pictures can include to the richness of flow cytometry information. Such as, the determine exhibits an usually standard apoptosis assay using Annexin V and PI, adding mobile imaging to characterize cells in Each and every population to expose morphologically distinctive features. These insights could not have been received from multiplex staining alone.

Whether or not you’re a rookie or a specialist, investigate our sources to progress your investigate and experience in flow cytometry.

Blend acoustic focusing technological know-how with large-parameter Evaluation for flexible spectral unmixing and traditional workflows.

Learn new insights into your experimental data by visualizing populations utilizing put together fluorescence and morphology data. Our software interface wizards permit downsampling, concatenation (merged data files) and dimensionality equipment which are created in to the program for an intuitive, streamlined information Investigation encounter.

"An image just isn't fairly truly worth one thousand dots, nonetheless it seriously helps to see photographs. It is great to find out images that correlate with staining. By way of example, AnnexinV-favourable cells which have been morphologically different than balanced cells, or CD14+ monocytes that are larger sized plus much more textured than CD3+/CD4+/CD14- lymphocytes.

In addition, it can be illuminating to see the number of doublets slip as a result of common singlet gates, and great to verify the standing of unpredicted double-optimistic situations in my Evaluation."

Pair your Attune flow cytometer using an autosampler for improved workflow effectiveness, and add robotic integration for maximum throughput.

Regular graphic excellent even at large throughput Acoustic concentrating as well as a superior-speed digicam Mix to image these T website cells constantly at lower or significant flow premiums. Very easily modify target and digicam settings to satisfy experimental demands.

The Attune NxT cytometer is the reliable workhorse instrument your lab can count on for steady and trustworthy functionality. The Attune CytPix product includes a higher-pace brightfield camera and AI-pushed picture Assessment program, with latest updates together with device Studying equipment and an intuitive consumer interface, enabling Improved facts precision, accuracy, and quicker insights into sample biology.

Probably most importantly, these events have cells of Plainly distinctive phenotypes which may lead to incorrect conclusions with regards to double favourable situations (especially in rare populations).

AI-driven automated image Examination with the Attune Cytometric Program interprets party attributes into unique morphology parameters that can be combined with typical fluorescence and scatter parameters to reveal hugely reproducible population alterations and one of a kind subsets.

Application Observe: Automatic picture Investigation lessens user-to-consumer variability in flow cytometry gating methods

Constructed-in dimensionality reduction resources improved resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to verify envisioned populace resolution in lysed whole human blood. Consumer can look at gatherings from Every cluster for visual affirmation of cell phenotypes.

No clean/no lyse leukocyte Investigation with imaging. Cells had been acquired from 24-hour-old blood by dilution in one mM EDTA ( Pro UX/UI permits customers to prepare AI designs for expanded purposes. (A) The “Prepare Product” attribute offers annotation tools and genuine-time design teaching accuracy.

Gating on these extended parameters allows you to immediately and properly establish populations of fascination and make sure gating tactic with little if any handbook overview. The Attune software program provides usage of condition-of-the-art equipment Finding out Evaluation resources within just an expertly made user interface, making it extremely user friendly.

As the mobile line had previously been analyzed on the Attune CytPix Flow Cytometer, the scientists went back again to the images and ended up able to doc the microbial an infection a minimum of 5 times earlier.

Agilent Price Guarantee assures you a minimum of a decade of instrument use from the date you buy. Or else, We're going to credit you Together with the residual worth of the technique towards an upgraded design.

In this article, we exhibit that by gating methods using graphic-centered quantitative parameters can distinguish interacting cells from coincident activities to far more precisely review interacting cells.